Reconstituted epithelial corneal tissues for evaluation of drug delivery

Authors

  • Susi Burgalassi Department of Pharmacy, University of Pisa, Pisa; Italian inter-university centre for the promotion of the 3Rs in teaching and research, Pisa
  • Erica Zucchetti Department of Pharmacy, University of Pisa, Pisa; Italian inter-university centre for the promotion of the 3Rs in teaching and research, Pisa
  • Eleonora Terreni Department of Pharmacy, University of Pisa, Pisa; Italian inter-university centre for the promotion of the 3Rs in teaching and research, Pisa
  • Daniela Monti Department of Pharmacy, University of Pisa, Pisa; Italian inter-university centre for the promotion of the 3Rs in teaching and research, Pisa
  • Silvia Tampucci Department of Pharmacy, University of Pisa, Pisa; Italian inter-university centre for the promotion of the 3Rs in teaching and research, Pisa
  • Patrizia Chetoni Department of Pharmacy, University of Pisa, Pisa; Italian inter-university centre for the promotion of the 3Rs in teaching and research, Pisa

DOI:

https://doi.org/10.4081/bse.82

Keywords:

Corneal permeation, reconstituted tissues, drug partition coefficient

Abstract

One possible approach to reduce the use of animals in the evaluation of the ocular drug delivery is that to use cell cultures as model of tissues. In this study are compared two different reconstituted epithelial corneal tissues, a homemade artificial corneal epithelium (Reconstituted Rabbit Corneal Epithelium) and a commercial human corneal epithelial model (COR-100 EpiCornealTM, MatTek), for permeation experiments of three drugs with different physical chemical properties: timolol maleate, cyclosporin A, and a newly synthesized compound, MAGL 17.b. The collected data show that corneal epithelial models are not sufficient to simulate the complexity of the corneal barrier and the presence of a layer simulating the stroma may be necessary to approach the structure of native cornea.

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Published

11-02-2020

Issue

Vol. 1 No. 1 (2020)

Section

Original Articles

License

Copyright (c) 2019 The Authors

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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How to Cite

Reconstituted epithelial corneal tissues for evaluation of drug delivery. (2020). Biomedical Science and Engineering, 1(1). https://doi.org/10.4081/bse.82