An in vitro enterocyte cell-like model to study Group B streptococci internalization

Authors

  • Giuseppe Valerio De Gaetano Department of Human Pathology, University of Messina, Messina; Scylla Biotech Srl, Messina
  • Germana Lentini Department of Human Pathology, University of Messina, Messina
  • Roberta Galbo Department of Chemical, Biological and Pharmaceutical Sciences, University of Messina, Messina
  • Francesco Coppolino Department of Human Pathology, University of Messina, Messina
  • Agata Famà Department of Human Pathology, University of Messina, Messina
  • Giuseppe Teti Department of Human Pathology, University of Messina, Messina; Scylla Biotech Srl, Messina

DOI:

https://doi.org/10.4081/bse.167

Keywords:

Caco-2 cells, GBS, actin, endocytosis

Abstract

We used a 2D in vitro growth model of Caco2 cells to study invasion of Streptococcus agalactiae. This model can be useful for evaluating the virulence of the most relevant enteric pathogens.

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References

Hon KL, Chan KH, Ko PL, et al. Late Onset Streptococcus agalactiae Meningitis following Early Onset Septicemia: A Preventable Disease? Case Rep Ped 2017;2017:8418105.

Snoeck V, Goddeeris B, Cox E. The role of enterocytes in the intestinal barrier function and antigen uptake. Microbes Infect 2005;7:997-1004.

Natoli M, Leoni BD, D'Agnano I, et al. Cell growing density affects the structural and functional properties of Caco2 differentiated monolayer. J Cell Physiol 2011;226:1531-43.

De Gaetano GV, Pietrocola G, Romeo L, et al. The Streptococcus agalactiae cell wall-anchored protein PbsP mediates adhesion to and invasion of epithelial cells by exploiting the host vitronectin/av integrin axis. Mol Microbiol2018;110:82-94.

Zaas DW, Swan ZD, Brown BJ, et al. Counteracting signaling activities in lipid rafts associated with the invasion of lung epithelial cells by Pseudomonas aeruginosa. J Biol Chem 2009;284:9955-64.

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Published

29-09-2021

Issue

Vol. 2 No. 1 (2021)

Section

Communications

License

Copyright (c) 2021 The Authors

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.

How to Cite

An in vitro enterocyte cell-like model to study Group B streptococci internalization. (2021). Biomedical Science and Engineering, 2(1). https://doi.org/10.4081/bse.167