Optimization of primary hepatocyte isolation for the pharmacological characterization of metabotropic glutamate receptor (mGluR) subtype 5: a study on Reduction

Authors

  • Clarissa Berardo Department of Internal Medicine and Therapeutics, University of Pavia, Pavia
  • Andrea Ferrigno Department of Internal Medicine and Therapeutics, University of Pavia, Pavia
  • Veronica Siciliano Department of Internal Medicine and Therapeutics, University of Pavia, Pavia
  • Marta Cagna Department of Internal Medicine and Therapeutics, University of Pavia, Pavia
  • Plinio Richelmi Department of Internal Medicine and Therapeutics, University of Pavia, Pavia
  • Mariapia Vairetti Department of Internal Medicine and Therapeutics, University of Pavia, Pavia
  • Laura G. Di Pasqua Department of Internal Medicine and Therapeutics, University of Pavia, Pavia

DOI:

https://doi.org/10.4081/bse.151

Keywords:

Reduction, mGluR5, collagenase, rat hepatocytes, MPEP

Abstract

To minimize the number of animals used during experiments, it is important to choose the suitable enzyme according to the final goal. In our work, we demonstrated the superiority of collagenase IV in the maintenance of functional transmembrane receptor, thus the pharmacological activity, in isolated rat hepatocytes.

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Published

29-09-2021

Issue

Vol. 2 No. 1 (2021)

Section

Communications

License

Copyright (c) 2021 The Authors

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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How to Cite

Optimization of primary hepatocyte isolation for the pharmacological characterization of metabotropic glutamate receptor (mGluR) subtype 5: a study on Reduction. (2021). Biomedical Science and Engineering, 2(1). https://doi.org/10.4081/bse.151